C-FLUSH: A novel cerebrospinal fluid-based therapeutic strategy to enhance brain-wide waste clearance

Glymphatic system and its manipulations have been extensively characterized in rodent models. The overall goal of this study is to implement C-FLUSH therapy to enhance the brain-wide solute clearance in a higher mammal to enable its clinical therapeutic translation. Herein, we aim to optimize and characterize the efficacy of C-FLUSH on fluorescently tagged amyloid-beta or related agent introduced into the cleaning system (CSF) or brain parenchyma and assess it clearance to the periphery.